diff --git a/tests/suites/test_suite_psa_crypto_init.data b/tests/suites/test_suite_psa_crypto_init.data
index 6996c115be..c57a764efe 100644
--- a/tests/suites/test_suite_psa_crypto_init.data
+++ b/tests/suites/test_suite_psa_crypto_init.data
@@ -1,3 +1,6 @@
+Create NV seed file
+create_nv_seed:
+
 PSA init/deinit
 init_deinit:2
 
@@ -48,3 +51,6 @@ entropy_from_nv_seed:MBEDTLS_ENTROPY_BLOCK_SIZE - 1:PSA_ERROR_INSUFFICIENT_ENTRO
 
 NV seed only: just enough
 entropy_from_nv_seed:ENTROPY_MIN_NV_SEED_SIZE:PSA_SUCCESS
+
+Recreate NV seed file
+create_nv_seed:
diff --git a/tests/suites/test_suite_psa_crypto_init.function b/tests/suites/test_suite_psa_crypto_init.function
index f4bb86f095..3596504298 100644
--- a/tests/suites/test_suite_psa_crypto_init.function
+++ b/tests/suites/test_suite_psa_crypto_init.function
@@ -127,6 +127,14 @@ static void custom_entropy_init( mbedtls_entropy_context *ctx )
  * END_DEPENDENCIES
  */
 
+/* BEGIN_CASE depends_on:MBEDTLS_ENTROPY_NV_SEED */
+void create_nv_seed( )
+{
+    static unsigned char seed[ENTROPY_MIN_NV_SEED_SIZE];
+    TEST_ASSERT( mbedtls_nv_seed_write( seed, sizeof( seed ) ) >= 0 );
+}
+/* END_CASE */
+
 /* BEGIN_CASE */
 void init_deinit( int count )
 {