diff --git a/data/pref.xml b/data/pref.xml
index bc9f500bf..66332f569 100644
--- a/data/pref.xml
+++ b/data/pref.xml
@@ -121,6 +121,11 @@
       <value id="HSV" value="1" />
       <value id="HSL" value="2" />
     </enum>
+    <enum id="SequenceDecision">
+      <value id="ASK" value="0" />
+      <value id="YES" value="1" />
+      <value id="NO" value="2" />
+    </enum>
   </types>
 
   <global>
@@ -313,6 +318,9 @@
     <section id="advanced_mode">
       <option id="show_alert" type="bool" default="true" />
     </section>
+    <section id="open_file">
+      <option id="open_sequence" type="SequenceDecision" default="SequenceDecision::ASK" />
+    </section>
     <section id="save_file">
       <option id="show_file_format_doesnt_support_alert" type="bool" default="true" />
       <option id="show_export_animation_in_sequence_alert" type="bool" default="true" />
diff --git a/data/strings/en.ini b/data/strings/en.ini
index 24207e5ca..63f08ea45 100644
--- a/data/strings/en.ini
+++ b/data/strings/en.ini
@@ -1,5 +1,5 @@
 # Aseprite
-# Copyright (C) 2018-2020  Igara Studio S.A.
+# Copyright (C) 2018-2021  Igara Studio S.A.
 # Copyright (C) 2016-2018  David Capello
 
 [advanced_mode]
@@ -1228,6 +1228,10 @@ the current frame & layer will be modified
 to focus the undid/redid change.
 END
 undo_allow_nonlinear_history = Allow non-linear history
+open_sequence_alert = Open a sequence of static files as an animation
+open_sequence_alert_ask = Ask
+open_sequence_alert_no = No
+open_sequence_alert_yes = Yes
 file_format_doesnt_support_alert = Show warning when saving a file with unsupported features
 export_animation_in_sequence_alert = Show warning when saving an animation as a sequence of static images
 overwrite_files_on_export_alert = Show warning when overwriting files on File > Export
diff --git a/data/widgets/open_sequence.xml b/data/widgets/open_sequence.xml
index 857f25ee8..be47668f0 100644
--- a/data/widgets/open_sequence.xml
+++ b/data/widgets/open_sequence.xml
@@ -1,4 +1,5 @@
 <!-- Aseprite -->
+<!-- Copyright (C) 2021 by Igara Studio S.A. -->
 <!-- Copyright (C) 2016 by David Capello -->
 <gui>
 <window id="open_sequence" text="@.title">
@@ -9,6 +10,7 @@
     </view>
     <separator horizontal="true" />
     <check id="repeat" text="@.repeat" />
+    <check id="dont_show" text="@general.dont_show" />
     <hbox>
       <boxfiller />
       <hbox homogeneous="true">
diff --git a/data/widgets/options.xml b/data/widgets/options.xml
index b810f59fd..6a2dc27be 100644
--- a/data/widgets/options.xml
+++ b/data/widgets/options.xml
@@ -426,6 +426,14 @@
         <!-- Alerts -->
         <vbox id="section_alerts">
           <separator text="@.section_alerts" horizontal="true" />
+          <hbox>
+            <label text="@.open_sequence_alert" />
+            <combobox id="open_sequence">
+              <listitem text="@.open_sequence_alert_ask" value="0" />
+              <listitem text="@.open_sequence_alert_yes" value="1" />
+              <listitem text="@.open_sequence_alert_no" value="2" />
+            </combobox>
+          </hbox>
           <check id="file_format_doesnt_support_alert" text="@.file_format_doesnt_support_alert"
                  pref="save_file.show_file_format_doesnt_support_alert" />
           <check id="export_animation_in_sequence_alert" text="@.export_animation_in_sequence_alert"
diff --git a/src/app/commands/cmd_open_file.cpp b/src/app/commands/cmd_open_file.cpp
index a2d1aaf1a..04473312f 100644
--- a/src/app/commands/cmd_open_file.cpp
+++ b/src/app/commands/cmd_open_file.cpp
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2019-2020  Igara Studio S.A.
+// Copyright (C) 2019-2021  Igara Studio S.A.
 // Copyright (C) 2001-2018  David Capello
 //
 // This program is distributed under the terms of
@@ -78,7 +78,7 @@ OpenFileCommand::OpenFileCommand()
   : Command(CommandId::OpenFile(), CmdRecordableFlag)
   , m_repeatCheckbox(false)
   , m_oneFrame(false)
-  , m_seqDecision(SequenceDecision::Ask)
+  , m_seqDecision(gen::SequenceDecision::ASK)
 {
 }
 
@@ -90,12 +90,18 @@ void OpenFileCommand::onLoadParams(const Params& params)
   m_oneFrame = params.get_as<bool>("oneframe");
 
   std::string sequence = params.get("sequence");
-  if (m_oneFrame || sequence == "skip")
-    m_seqDecision = SequenceDecision::Skip;
-  else if (sequence == "agree")
-    m_seqDecision = SequenceDecision::Agree;
-  else
-    m_seqDecision = SequenceDecision::Ask;
+  if (m_oneFrame ||
+      sequence == "skip" ||
+      sequence == "no") {
+    m_seqDecision = gen::SequenceDecision::NO;
+  }
+  else if (sequence == "agree" ||
+           sequence == "yes") {
+    m_seqDecision = gen::SequenceDecision::YES;
+  }
+  else {
+    m_seqDecision = gen::SequenceDecision::ASK;
+  }
 }
 
 void OpenFileCommand::onExecute(Context* context)
@@ -143,14 +149,25 @@ void OpenFileCommand::onExecute(Context* context)
     FILE_LOAD_CREATE_PALETTE |
     (m_repeatCheckbox ? FILE_LOAD_SEQUENCE_ASK_CHECKBOX: 0);
 
+  if (context->isUIAvailable() &&
+      m_seqDecision == gen::SequenceDecision::ASK) {
+    if (Preferences::instance().openFile.openSequence() == gen::SequenceDecision::ASK) {
+      // Do nothing (ask by default, or whatever the command params
+      // specified)
+    }
+    else {
+      m_seqDecision = Preferences::instance().openFile.openSequence();
+    }
+  }
+
   switch (m_seqDecision) {
-    case SequenceDecision::Ask:
+    case gen::SequenceDecision::ASK:
       flags |= FILE_LOAD_SEQUENCE_ASK;
       break;
-    case SequenceDecision::Agree:
+    case gen::SequenceDecision::YES:
       flags |= FILE_LOAD_SEQUENCE_YES;
       break;
-    case SequenceDecision::Skip:
+    case gen::SequenceDecision::NO:
       flags |= FILE_LOAD_SEQUENCE_NONE;
       break;
   }
@@ -179,12 +196,12 @@ void OpenFileCommand::onExecute(Context* context)
     else {
       if (fop->isSequence()) {
         if (fop->sequenceFlags() & FILE_LOAD_SEQUENCE_YES) {
-          m_seqDecision = SequenceDecision::Agree;
+          m_seqDecision = gen::SequenceDecision::YES;
           flags &= ~FILE_LOAD_SEQUENCE_ASK;
           flags |= FILE_LOAD_SEQUENCE_YES;
         }
         else if (fop->sequenceFlags() & FILE_LOAD_SEQUENCE_NONE) {
-          m_seqDecision = SequenceDecision::Skip;
+          m_seqDecision = gen::SequenceDecision::NO;
           flags &= ~FILE_LOAD_SEQUENCE_ASK;
           flags |= FILE_LOAD_SEQUENCE_NONE;
         }
diff --git a/src/app/commands/cmd_open_file.h b/src/app/commands/cmd_open_file.h
index cae919200..5437611f4 100644
--- a/src/app/commands/cmd_open_file.h
+++ b/src/app/commands/cmd_open_file.h
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2020  Igara Studio S.A.
+// Copyright (C) 2020-2021  Igara Studio S.A.
 // Copyright (C) 2016-2018  David Capello
 //
 // This program is distributed under the terms of
@@ -10,6 +10,7 @@
 #pragma once
 
 #include "app/commands/command.h"
+#include "app/pref/preferences.h"
 #include "base/paths.h"
 
 #include <string>
@@ -18,17 +19,13 @@ namespace app {
 
   class OpenFileCommand : public Command {
   public:
-    enum class SequenceDecision {
-      Ask, Agree, Skip,
-    };
-
     OpenFileCommand();
 
     const base::paths& usedFiles() const {
       return m_usedFiles;
     }
 
-    SequenceDecision seqDecision() const {
+    gen::SequenceDecision seqDecision() const {
       return m_seqDecision;
     }
 
@@ -42,7 +39,7 @@ namespace app {
     bool m_repeatCheckbox;
     bool m_oneFrame;
     base::paths m_usedFiles;
-    SequenceDecision m_seqDecision;
+    gen::SequenceDecision m_seqDecision;
   };
 
 } // namespace app
diff --git a/src/app/commands/cmd_options.cpp b/src/app/commands/cmd_options.cpp
index e84a3c1c5..a1d0cd664 100644
--- a/src/app/commands/cmd_options.cpp
+++ b/src/app/commands/cmd_options.cpp
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2018-2020  Igara Studio S.A.
+// Copyright (C) 2018-2021  Igara Studio S.A.
 // Copyright (C) 2001-2018  David Capello
 //
 // This program is distributed under the terms of
@@ -235,6 +235,7 @@ public:
     }
 
     // Alerts
+    openSequence()->setSelectedItemIndex(int(m_pref.openFile.openSequence()));
     resetAlerts()->Click.connect([this]{ onResetAlerts(); });
 
     // Cursor
@@ -681,6 +682,9 @@ public:
     m_curPref->bg.color1(checkedBgColor1()->getColor());
     m_curPref->bg.color2(checkedBgColor2()->getColor());
 
+    // Alerts preferences
+    m_pref.openFile.openSequence(gen::SequenceDecision(openSequence()->getSelectedItemIndex()));
+
     int undo_size_limit_value;
     undo_size_limit_value = undoSizeLimit()->textInt();
     undo_size_limit_value = base::clamp(undo_size_limit_value, 0, 999999);
diff --git a/src/app/file/file.cpp b/src/app/file/file.cpp
index bb4444e22..39f2ffc75 100644
--- a/src/app/file/file.cpp
+++ b/src/app/file/file.cpp
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2018-2020  Igara Studio S.A.
+// Copyright (C) 2018-2021  Igara Studio S.A.
 // Copyright (C) 2001-2018  David Capello
 //
 // This program is distributed under the terms of
@@ -261,10 +261,19 @@ FileOp* FileOp::createLoadDocumentOperation(Context* context,
 
         window.openWindowInForeground();
 
+        // Don't show this alert again.
+        if (window.dontShow()->isSelected()) {
+          Preferences::instance().openFile.openSequence(
+            window.closer() == window.agree() ?
+              gen::SequenceDecision::YES:
+              gen::SequenceDecision::NO);
+        }
+
         // If the user selected the "do the same for other files"
         // checkbox, we've to save what the user want to do for the
         // following files.
-        if (window.repeat()->isSelected()) {
+        if (window.repeat()->isSelected() ||
+            window.dontShow()->isSelected()) {
           if (window.closer() == window.agree())
             fop->m_seq.flags = FILE_LOAD_SEQUENCE_YES;
           else
diff --git a/src/app/script/values.cpp b/src/app/script/values.cpp
index 311356834..ddf14855a 100644
--- a/src/app/script/values.cpp
+++ b/src/app/script/values.cpp
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2019-2020  Igara Studio S.A.
+// Copyright (C) 2019-2021  Igara Studio S.A.
 //
 // This program is distributed under the terms of
 // the End-User License Agreement for Aseprite.
@@ -174,11 +174,12 @@ FOR_ENUM(app::gen::PivotPosition)
 FOR_ENUM(app::gen::PixelConnectivity)
 FOR_ENUM(app::gen::RightClickMode)
 FOR_ENUM(app::gen::SelectionMode)
+FOR_ENUM(app::gen::SequenceDecision)
 FOR_ENUM(app::gen::StopAtGrid)
 FOR_ENUM(app::gen::SymmetryMode)
 FOR_ENUM(app::gen::TimelinePosition)
-FOR_ENUM(app::gen::WindowColorProfile)
 FOR_ENUM(app::gen::ToGrayAlgorithm)
+FOR_ENUM(app::gen::WindowColorProfile)
 FOR_ENUM(app::tools::FreehandAlgorithm)
 FOR_ENUM(app::tools::RotationAlgorithm)
 FOR_ENUM(doc::AniDir)
diff --git a/src/app/util/open_batch.h b/src/app/util/open_batch.h
index 39b43bc25..c2e611348 100644
--- a/src/app/util/open_batch.h
+++ b/src/app/util/open_batch.h
@@ -1,5 +1,5 @@
 // Aseprite
-// Copyright (C) 2020  Igara Studio S.A.
+// Copyright (C) 2020-2021  Igara Studio S.A.
 //
 // This program is distributed under the terms of
 // the End-User License Agreement for Aseprite.
@@ -29,14 +29,14 @@ namespace app {
         params.set("oneframe", "true");
       else {
         switch (m_lastDecision) {
-          case OpenFileCommand::SequenceDecision::Ask:
+          case gen::SequenceDecision::ASK:
             params.set("sequence", "ask");
             params.set("repeat_checkbox", "true");
             break;
-          case OpenFileCommand::SequenceDecision::Skip:
+          case gen::SequenceDecision::NO:
             params.set("sequence", "skip");
             break;
-          case OpenFileCommand::SequenceDecision::Agree:
+          case gen::SequenceDecision::YES:
             params.set("sequence", "agree");
             break;
         }
@@ -49,7 +49,7 @@ namespace app {
 
       // Future decision for other files in the CLI
       auto d = m_cmd.seqDecision();
-      if (d != OpenFileCommand::SequenceDecision::Ask)
+      if (d != gen::SequenceDecision::ASK)
         m_lastDecision = d;
     }
 
@@ -59,7 +59,7 @@ namespace app {
 
   private:
     OpenFileCommand m_cmd;
-    OpenFileCommand::SequenceDecision m_lastDecision = OpenFileCommand::SequenceDecision::Ask;
+    gen::SequenceDecision m_lastDecision = gen::SequenceDecision::ASK;
   };
 
 } // namespace app