diff --git a/data/gui.xml b/data/gui.xml
index 08066ea71..a213b7e6e 100644
--- a/data/gui.xml
+++ b/data/gui.xml
@@ -303,6 +303,12 @@
       <key command="LayerOpacity"><param name="opacity" value="224" /></key>
       <key command="LayerOpacity" shortcut="Shift+4"><param name="opacity" value="255" /></key>
 
+      <key command="ToggleOtherLayersOpacity" />
+      <key command="ToggleOtherLayersOpacity" shortcut="Shift+F7">
+        <param name="preview" value="true" />
+        <param name="checkedIfZero" value="true" />
+      </key>
+
       <!-- Scroll to center -->
       <key command="ScrollCenter" shortcut="Shift+Z" />
       <key command="FitScreen" shortcut="Ctrl+0" mac="Cmd+0" />
@@ -1017,7 +1023,14 @@
         <item command="Timeline" text="@.view_timeline">
           <param name="switch" value="true" />
         </item>
-        <item command="TogglePreview" text="@.view_preview" />
+        <menu text="@.view_preview">
+          <item command="TogglePreview" text="@.view_preview" />
+          <separator />
+          <item command="ToggleOtherLayersOpacity" text="@.view_preview_hide_other_layers">
+            <param name="preview" value="true" />
+            <param name="checkedIfZero" value="true" />
+          </item>
+        </menu>
         <item command="AdvancedMode" text="@.view_advanced_mode" />
         <item command="FullscreenMode" text="@.view_full_screen_mode" />
         <item command="FullscreenPreview" text="@.view_full_screen_preview" />
diff --git a/data/pref.xml b/data/pref.xml
index 16f1110f7..9b92ebea5 100644
--- a/data/pref.xml
+++ b/data/pref.xml
@@ -234,6 +234,7 @@
       <option id="load_wintab_driver" type="bool" default="false" />
       <option id="flash_layer" type="bool" default="false" />
       <option id="nonactive_layers_opacity" type="int" default="255" />
+      <option id="nonactive_layers_opacity_preview" type="int" default="255" />
     </section>
     <section id="news">
       <option id="cache_file" type="std::string" />
diff --git a/data/strings/en.ini b/data/strings/en.ini
index 78bcb0594..81c8bf733 100644
--- a/data/strings/en.ini
+++ b/data/strings/en.ini
@@ -601,6 +601,8 @@ SwitchNonactiveLayersOpacity = Switch Nonactive Layers Opacity
 SymmetryMode = Symmetry Mode
 TiledMode = Tiled Mode
 Timeline = Switch Timeline
+ToggleOtherLayersOpacity = Toggle Other Layers Opacity
+ToggleOtherLayersOpacity_PreviewEditor = Toggle Other Layers Opacity in Preview
 TogglePlayAll = Play All Frames (Ignore Tags)
 TogglePlayOnce = Play Once
 TogglePlaySubtags = Play Subtags & Repetitions
@@ -1350,6 +1352,7 @@ view_set_loop_section = Set &Loop Section
 view_show_onion_skin = Show &Onion Skin
 view_timeline = &Timeline
 view_preview = Previe&w
+view_preview_hide_other_layers = &Hide Other Layers
 view_advanced_mode = &Advanced Mode
 view_full_screen_mode = &Full Screen Mode
 view_full_screen_preview = F&ull Screen Preview
diff --git a/src/app/CMakeLists.txt b/src/app/CMakeLists.txt
index 4d56d765f..12aac8278 100644
--- a/src/app/CMakeLists.txt
+++ b/src/app/CMakeLists.txt
@@ -330,6 +330,7 @@ if(ENABLE_UI)
     commands/set_playback_speed.cpp
     commands/show_menu.cpp
     commands/tileset_mode.cpp
+    commands/toggle_other_layers_opacity.cpp
     commands/toggle_play_option.cpp
     file_selector.cpp
     modules/gfx.cpp
diff --git a/src/app/commands/commands_list.h b/src/app/commands/commands_list.h
index cd1cb78d4..4b732b903 100644
--- a/src/app/commands/commands_list.h
+++ b/src/app/commands/commands_list.h
@@ -168,6 +168,7 @@ FOR_EACH_COMMAND(SymmetryMode)
 FOR_EACH_COMMAND(TiledMode)
 FOR_EACH_COMMAND(TilesetMode)
 FOR_EACH_COMMAND(Timeline)
+FOR_EACH_COMMAND(ToggleOtherLayersOpacity)
 FOR_EACH_COMMAND(TogglePlayAll)
 FOR_EACH_COMMAND(TogglePlayOnce)
 FOR_EACH_COMMAND(TogglePlaySubtags)
diff --git a/src/app/commands/toggle_other_layers_opacity.cpp b/src/app/commands/toggle_other_layers_opacity.cpp
new file mode 100644
index 000000000..ea670c939
--- /dev/null
+++ b/src/app/commands/toggle_other_layers_opacity.cpp
@@ -0,0 +1,114 @@
+// Aseprite
+// Copyright (C) 2023  Igara Studio S.A.
+//
+// This program is distributed under the terms of
+// the End-User License Agreement for Aseprite.
+
+#ifdef HAVE_CONFIG_H
+#include "config.h"
+#endif
+
+#include "app/app.h"
+#include "app/commands/new_params.h"
+#include "app/i18n/strings.h"
+#include "app/ui/editor/editor.h"
+#include "app/ui/main_window.h"
+#include "app/ui/preview_editor.h"
+#include "fmt/format.h"
+#include "ui/system.h"
+
+namespace app {
+
+struct ToggleOtherLayersOpacityParams : public NewParams {
+  Param<bool> preview { this, false, "preview" };
+  Param<int> opacity { this, 255, "opacity" };
+  // Can be used if the option is presend as "Hide Others", so it's
+  // checked when the configured opacity == 0
+  Param<bool> checkedIfZero { this, false, "checkedIfZero" };
+};
+
+class ToggleOtherLayersOpacityCommand : public CommandWithNewParams<ToggleOtherLayersOpacityParams> {
+public:
+  ToggleOtherLayersOpacityCommand();
+private:
+  bool onChecked(Context* ctx) override;
+  void onExecute(Context* ctx) override;
+  std::string onGetFriendlyName() const override;
+};
+
+ToggleOtherLayersOpacityCommand::ToggleOtherLayersOpacityCommand()
+  : CommandWithNewParams<ToggleOtherLayersOpacityParams>(
+    CommandId::ToggleOtherLayersOpacity(), CmdUIOnlyFlag)
+{
+}
+
+bool ToggleOtherLayersOpacityCommand::onChecked(Context* ctx)
+{
+  if (params().checkedIfZero.isSet()) {
+    auto& pref = Preferences::instance();
+    auto& option =
+      (params().preview() ?
+       pref.experimental.nonactiveLayersOpacityPreview:
+       pref.experimental.nonactiveLayersOpacity);
+    return (option() == 0);
+  }
+  return false;
+}
+
+void ToggleOtherLayersOpacityCommand::onExecute(Context* ctx)
+{
+  auto& pref = Preferences::instance();
+  auto& option =
+    (params().preview() ?
+     pref.experimental.nonactiveLayersOpacityPreview:
+     pref.experimental.nonactiveLayersOpacity);
+
+  // If we want to toggle the other layers in the preview window, and
+  // the preview window is hidden, we just show it with the "other
+  // layers" hidden.
+  if (params().preview()) {
+    PreviewEditorWindow* previewWin =
+      App::instance()->mainWindow()->getPreviewEditor();
+    if (!previewWin->isPreviewEnabled()) {
+      option(0);
+      previewWin->setPreviewEnabled(true);
+      return;
+    }
+  }
+
+  if (params().opacity.isSet()) {
+    option(params().opacity());
+  }
+  else {
+    option(option() == 0 ? 255: 0);
+  }
+
+  // TODO make the editors listen the opacity change
+  if (params().preview()) {
+    PreviewEditorWindow* previewWin =
+      App::instance()->mainWindow()->getPreviewEditor();
+    previewWin->previewEditor()->invalidate();
+  }
+  else {
+    app_refresh_screen();
+  }
+}
+
+std::string ToggleOtherLayersOpacityCommand::onGetFriendlyName() const
+{
+  std::string text;
+  if (params().preview())
+    text = Strings::commands_ToggleOtherLayersOpacity_PreviewEditor();
+  else
+    text = Strings::commands_ToggleOtherLayersOpacity();
+  if (params().opacity.isSet())
+    text = fmt::format("{} ({})", text, params().opacity());
+  return text;
+}
+
+Command* CommandFactory::createToggleOtherLayersOpacityCommand()
+{
+  return new ToggleOtherLayersOpacityCommand;
+}
+
+} // namespace app
diff --git a/src/app/ui/editor/editor.cpp b/src/app/ui/editor/editor.cpp
index 616e2954e..63478f6c1 100644
--- a/src/app/ui/editor/editor.cpp
+++ b/src/app/ui/editor/editor.cpp
@@ -383,7 +383,7 @@ void Editor::setLayer(const Layer* layer)
         // If the user want to see the active layer edges...
         m_docPref.show.layerEdges() ||
         // If there is a different opacity for nonactive-layers
-        Preferences::instance().experimental.nonactiveLayersOpacity() < 255 ||
+        otherLayersOpacity() < 255 ||
         // If the automatic cel guides are visible...
         m_showGuidesThisCel ||
         // If grid settings changed
@@ -672,10 +672,7 @@ void Editor::drawOneSpriteUnclippedRect(ui::Graphics* g, const gfx::Rect& sprite
     m_renderEngine->setNewBlendMethod(pref.experimental.newBlend());
     m_renderEngine->setRefLayersVisiblity(true);
     m_renderEngine->setSelectedLayer(m_layer);
-    if (m_flags & Editor::kUseNonactiveLayersOpacityWhenEnabled)
-      m_renderEngine->setNonactiveLayersOpacity(pref.experimental.nonactiveLayersOpacity());
-    else
-      m_renderEngine->setNonactiveLayersOpacity(255);
+    m_renderEngine->setNonactiveLayersOpacity(otherLayersOpacity());
     m_renderEngine->setupBackground(m_document, IMAGE_RGB);
     m_renderEngine->disableOnionskin();
 
@@ -2978,9 +2975,18 @@ void Editor::updateAutoCelGuides(ui::Message* msg)
   }
 }
 
+int Editor::otherLayersOpacity() const
+{
+  if (m_docView && m_docView->isPreview())
+    return Preferences::instance().experimental.nonactiveLayersOpacityPreview();
+  else
+    return Preferences::instance().experimental.nonactiveLayersOpacity();
+}
+
 // static
 void Editor::registerCommands()
 {
+  // TODO merge with ToggleOtherLayersOpacity
   Commands::instance()
     ->add(
       new QuickCommand(
diff --git a/src/app/ui/editor/editor.h b/src/app/ui/editor/editor.h
index be23fb28e..e6830c6a7 100644
--- a/src/app/ui/editor/editor.h
+++ b/src/app/ui/editor/editor.h
@@ -90,15 +90,13 @@ namespace app {
       kShowDecorators = 16,
       kShowSymmetryLine = 32,
       kShowSlices = 64,
-      kUseNonactiveLayersOpacityWhenEnabled = 128,
       kDefaultEditorFlags = (kShowGrid |
                              kShowMask |
                              kShowOnionskin |
                              kShowOutside |
                              kShowDecorators |
                              kShowSymmetryLine |
-                             kShowSlices |
-                             kUseNonactiveLayersOpacityWhenEnabled)
+                             kShowSlices)
     };
 
     enum class ZoomBehavior {
@@ -398,6 +396,8 @@ namespace app {
     void invalidateIfActive();
     void updateAutoCelGuides(ui::Message* msg);
 
+    int otherLayersOpacity() const;
+
     // Stack of states. The top element in the stack is the current state (m_state).
     EditorStatesHistory m_statesHistory;
     EditorStatesHistory m_deletedStates;